Preparation of probiotic oil suspension containing Lactobacillus acidophilus

Tóm tắt

Probiotics are defined as microorganisms that, when administered in sufficient quantities, confer a health benefit on the host. As they are very susceptible to environmental conditions, solid dosage forms are used to increase their stability. In pediatric use, liquid preparations of edible oily suspensions containing probiotics are more favorable since they help overcome the difficulty of oral administration in children. However, few research articles related to the preparation process of these oily suspensions were reported in Vietnam. Our study focuses on preparing an oily suspension containing L. acidophilus at a laboratory scale, oriented for use in infants and children under two years of age. The oily suspension was prepared using the dispersion method. Different mixtures of oil and dispersing agents were studied for their effect on the stability of the suspension in terms of sedimentation rate, drop time, average particle size, and the amount of L. acidophilus. The results showed that an oily suspension containing L. acidophilus could be made using the dispersion method with a mixture of oils as the dispersion medium and Aerosil and aluminum tristearate as dispersing agents. The prepared suspension had an appropriate sedimentation rate of about 10–20%, an average drop time of 1,0 drop/s, an average particle size of 3–3,5 µm, and an amount of L. acidophilus above 3 x 10⁸ - 5 x 10⁸ CFU/ml. Our study successfully prepared an oily suspension containing L. acidophilus using the dispersion method. The prepared suspension had good physical stability, and the number of L. acidophilus remained over 10⁸ CFU/ml during two months of storage.