Garlic extract (Allium Sativum L.) mitigates oleic acid-induced lipid accumulation and lipotoxicity in hepatocytes

Tóm tắt

Garlic extract has long been used in traditional medicine for managing liver disorders; however, its effects on hepatic steatogenesis remain unexplored. The study was conducted to evaluate the inhibitory effect on lipid accumulation of garlic extract in oleic acid-induced HepG2 hepatocytes. Pretreatment with GE at 30 μg/mL significantly abrogated the steatogenic effects of OA. Specifically, GE reduced the intracellular lipid accumulation, as evidenced by both Oil Red O staining and BODIPY fluorescence assays. Additionally, GE exerted dose-dependent inhibitory effects on OA-induced increases in cellular triglyceride levels. Garlic extract at 10 and 30 μg/mL exhibited strong antioxidant effects by reducing the formation of reactive oxygen species (ROS) and malondialdehyde (MDA) in oleic acid-induced HepG2 hepatocytes. Garlic extract at 30 μg/mL also protected hepatocytes from OA-induced damage, as evidenced by the restoration of cell viability, reductions in AST and ALT activity, and inhibition of apoptotic cell death through decreased expression of caspase-3. Taken together, the results indicated that garlic extract may have potential for managing hepatic steatosis